Allergeneye® ELISA Ⅱ Series
This series conforms to the guidelines in
"Testing Methods for Foods Containing Allergens"
(March 26, 2014, Shoshoku No. 36, Notification
by the Deputy Director-General of the Consumer Affairs Agency)
Food Allergen Simple Testing Kit
Allergeneye® ELISA Ⅱ Series
- egg, milk, wheat, buckwheat, peanut -
Allergeneye® ELISA Ⅱ
Features
Contains no toxic substances and is easy to handle
- Storage and disposal are the same as for general reagents and can be used safely.
High sensitivity measurement is possible
- Possible to measure from 0.78 ng/ml.
Possible to test various foods
- Tests a wide range of foods, from raw materials to processed foods.
Quantitative Kit
- This kit can be used for the determination of 1 to 20 ppm in food.
Excellent Specificity
- It uses monoclonal antibodies and has excellent specificity.
Kit Configuration
| Allergen Eye ELISA Ⅱ | |||
| Product Name | Capacity | Quantity | |
|---|---|---|---|
| A | Antibody-coated plate (with cover) (8-well x 12 rows) |
96-well | 1 sheet |
| B | Standard product (50 ng/mL) | 1mL | 2 bottles |
| C | Enzyme-labeled antibody fluid | 13mL | 1 bottle |
| D | Chromogenic solution (TMB solution) |
13mL | 1 bottle |
| E | Reaction stop solution (0.5 M hydrochloric acid, 0.25 M sulfuric acid) |
13mL | 1 bottle |
| F | Buffer solution for dilution | 100mL | 1 bottle |
| G | Washing liquid (10 times concentrated) |
100mL | 1 bottle |
| H | Liquid A for extraction (10 times concentrated liquid) |
100mL | 1 bottle |
| I | Liquid B for extraction (10 times concentrated liquid) |
100mL | 1 bottle |
| J | Liquid C for extraction (10 times concentrated liquid) |
100mL | 1 bottle |
| Instruction Manual | 1 copy | ||
Indicator protein
| Product Name | Indicator protein |
|---|---|
| Allergen Eye ELISA Ⅱ Egg | Ovalbumin |
| Allergen Eye ELISA Ⅱ Milk | Beta-lactoglobulin |
| Allergen Eye ELISA Ⅱ Wheat | Gliadin |
| Allergen Eye ELISA Ⅱ Buckwheat | 24kDa protein |
| Allergen Eye ELISA Ⅱ Peanut | Ara h 1 |
Measuring principle
 |
Antibody-coated plate |
|---|---|
 |
Primary reaction: Allergens bind to antibodies solidified on the plate. |
 |
Secondary reaction: The enzyme-labeled antibody binds to the allergens bound to the antibody on the plate in a sandwich-like fashion. |
 |
Enzymatic reaction: When an enzyme substrate is added, color is produced by enzymatic decomposition. The degree of this coloration is measured. |
 |
|
Testing Method
| Weigh out 1 g of food sample | Sample Extraction Operation |
|---|---|
| Add 19 mL of extraction reagent and extract by shaking(at least 12 hours at room temperature) | |
| Primary reaction (addition of standard solution and measurement solution) (1 hour at room temperature) | Measurement with ELISA |
| Secondary reaction (addition of enzyme-labeled antibody)(30 minutes at room temperature) | |
| Enzyme reaction (addition of chromogenic solution)(10 minutes at room temperature) | |
| Absorbance measurement |
Instruction Manual / Safety Data Sheet (SDS)
Correlation with Allergeneye, False Positives, and False Negatives
Product Code / Price
| Product Name | Product Code | Capacity | Base Price (excluding tax) |
|---|---|---|---|
| Allergen Eye ELISA Ⅱ Egg | 077834 | For 96 uses | 78,000 yen |
| Allergen Eye ELISA Ⅱ Milk | 077836 | ||
| Allergen Eye ELISA Ⅱ Wheat | 077847 | ||
| Allergen Eye ELISA Ⅱ Buckwheat | 077852 | ||
| Allergen Eye ELISA Ⅱ Peanut | 077860 |
*Allergeneye ELISA II Kiwifruit is made to order. For more information, please contact us.
Handling Precautions
This kit is a research reagent for detecting specific raw materials in food, and is not a clinical reagent for diagnosing the presence or absence of food allergies. The correlation between the amount of allergen intake and allergic symptoms is unknown.
Distributed by: R&D Center, Prima Meat Packers Co., Ltd.
635 Nakamukohara, Tsuchiura-shi, Ibaraki 300-0841, Japan
Tel：029-842-4333
Fax：029-842-5216
E-mail：Allergeneye@primaham.co.jp